网络棋牌

糖尿病药筛方案(小鼠版)

        糖尿病模型(DM)是胰岛素抵抗和胰岛素缺乏导致的以高血糖为主要特征表现的代谢紊乱综合征,易发生心、脑、肾等并发症。糖尿病状态下血浆游离脂肪酸异常增高,心肌耗能增加,葡萄糖代谢下降,脂肪酸代谢增加,心脏内过量的脂肪酸摄取和氧化导致心肌内脂肪代谢产物的积聚引起心脏脂质毒性,并在此基袖上出现氧化应激,导致细胞调亡、内皮功能紊乱、炎症反应增加,同时出现心肌的损伤,心肌的结构和功能均发生改变,最后导致糖尿病患者的死亡。

Ⅱ型小鼠造模及治疗方案

动物选取

SPF级Balb/C小鼠,雄性,周龄为4w~6w,体重为18g~20g。
经验:先适应性喂养一周,体内外环境适应,一周后体重体重水平20-24g.

高脂喂养
高脂喂养4-6周,体重达到32g以上。

高脂饲料

BJBAG1035DM (日粮型),BJBAG12451M(纯化型),同时设立空白组。

预试验阶段

1.YUSHIYANFENZU:

ZAOMOTIDUSHIYANFEN3ZU(LIRU:120mg/kg, 110mg/kg,100mg/kg)。

备注:预试剂量值选取,和批次体重水平相关。体重水平高,应调低单位给药量水平,不同品系和重量水平的给药推荐可以咨询我公司技术客服。

2.治疗份组:对照组(模型组)、阳性药组、受试药组(三个浓度)。

3.组只要求:建议每组10-15只动物。
网络棋牌   备注:统计学要求,组只10只以上,具备统计学意义。

4. SHIYANZHOUQI:SHIYINGXINGWEIYANG1w+MOXINGPANDING2w+ZHILIAO(4-12w)+QUCAI

STZ注射及缓冲液配置

网络棋牌5. NINGMENGSUANNAHUANCHONGYEPEIZHI:JIANG2.10 gNINGMENGSUANJIARUSHUANGZHENGSHUI100 mlPEICHENGNINGMENGSUANMUYE, CHENGWEIAYE; JIANG2.94gNINGMENGSUANSANNAJIARUSHUANGZHENGSHUI100 mlPEICHENGNINGMENGSUANNAMUYE,CHENGWEIBYE; JIANGA、BYEAN1:1BILI(HUO1:1.32)HUNHE,pHJICEDINGpHZHI,DIAODINGRONGYEpH=4-4.4ZHIJIAN,JISHISUOXUPEIZHISTZDE0.1 mol/LNINGMENGSUANNAHUANCHONGYE。

网络棋牌6. STZRONGYEDEPEIZHI:AN1%XISHISTZ, JI10mgSTZ:1mlSTZ,BINGYONG0.22μmLVJUNQIGUOLVCHUJUN。XIANYONGXIANPEI。

正式造模

7.KONGFU12h,JIANYIGEYEJINSHI;

8. MOXINGZUXIAOSHUANZHAOYUSHIYANMOSUODEJILIANGZHUSHESTZ, FUQIANGZHUSHEHUOJINGMAIZHUSHE。

9. STZZHUSHE3dHOU,CEKONGFUXUETANG,XUANZEXUETANGGAOYU16. 7 mmol/LNARUZHENGSHISHIYAN。

10. ZHILIAO(YAOWUGANYU)JIEDUAN,SHELIDUIZHAOZU,DUIZHAOZUYINGTONGBUZHUSHEYAOWUXISHIYE,MEIZHOUCEXUETANG、TIZHONG,GUANCHATIZHONGXUETANGBIANHUA。

取材阶段

11.ZHILIAOJIESHUHOU,GENJUCELIANGZHIDEXUQIU,XUANQUDUIYINGDEGUDINGFANGFAJINXINGQUCAI。QUHUANGZHI,PIXIAZHIFANG,GUGEJI,BAIZHI,GANZANG,FEIZANG,XINZANG,SHENZANGYONGYUBINGLIHEFENZISHENGWUXUEJIANCE,ZHAIYANQIUCAIXUE,SHIWENJINGZHI2hHOUYU4℃ 3000rLIXIN10FENZHONGTIQUXUEQING,FANGRU-80BINGXIANGDONGCUN。

模型评价

网络棋牌1. DATIGUANCHA:ZHENGCHANGZUXIAOSHUTIXINGSHIZHONG,JINGSHENZHUANGTAILIANGHAO,DONGZUOZIRU,FANYINGLINGMIN,MAOFAPINGFUYOUGUANGZE。ERTANGNIAOBINGXIAOSHUTIZHONGBIANQING,JINGSHENWEIMI,FANYINGCHIDUN,MAOSHUWUGUANGZE,DONGZUOCHIHUAN,GONGBEIJUANTI,NIAOLIANGXIANZHUZENGJIA。TANGNIAOBINGXIAOSHUJIAOZHENGCHANGZUTIZHONGXIANZHUJIANQING,XINZANGXISHU、GANZANGXISHUJIAOZHENGCHANGZUYOUXIANZHUZENGJIA,QIEYOUTONGJIXUEYIYI。

2. KONGFUXUETANG、GANYOUSANZHIHEZONGDANGUCHUNSHUIPINGDEBIANHUA:

网络棋牌ZHENGCHANGDUIZHAOZUDEKONGFUXUETANG、GANYOUSANZHIHEZONGDANGUCHUNSHUIPINGZAISHIYANQIANHOUJUNWUXIANZHUCHAYI;

网络棋牌MOXINGZUDEKONGFUXUETANG、GANYOUSANZHIHEZONGDANGUCHUNSHUIPINGXIANZHUGAOYUDUIZHAOZU,

3. TANGNIAOBINGXIAOSHUXINZANGZUZHIXINGTAIXUEDEBIANHUA

网络棋牌JINGHERANSEKEJIANZHENGCHANGDUIZHAOZUXIAOSHUXINJIXIANWEIPAILIEZHENGQI,ZHIMI,JIEGOUQINGXI,XIBAOHERANSEZHIFENBUJUNYUN,XIBAOWAIJIANZHIJIAOSHAO,WEIXUEGUANJIEGOUZHENGCHANG,KEJIANSHAOLIANGCHENGXIANWEIXIBAO,JIANZHIWUMINGXIANDEYANZHENGXIBAOJINRUN;TANGNIAOBINGMOXINGZUXIAOSHUXINJIXIANWEIPAILIEWENLUAN,XINJIXIBAOFEIDA,XIBAOJIANXIZENGDA,JIEGOUBUQINGXI,YOUDEXIBAOHEMOZHOUSUO,HEGUSUO,BINGYOUYANZHENGXIBAOJINRUN,JIXIBAOJIANYEKEJIANCHENGXIANWEIXIBAOJINRUN

4. TANGNIAOBINGXIAOSHUFEIZANGZUZHIXINGTAIXUEDEBIANHUA

HERANSEXIANSHI,DUIZHAOZUXIAOSHUFEIZUZHIJIEGOUQINGXI,FEIPAOFENBUJUNGOU,FEIPAOGE、ZHIQIGUAN

BIWANZHENG,FEIJIANZHIFENBUJUNYUN,SHAOJIANYANXINGXIBAOJINRUN;DMZUXIAOSHUFEIZUZHIJIEGOUWENLUAN,BUFENFEIPAOQIANGWEISUO、TAXIAN,KEJIANFEIDAPAO,ZHIQIGUANBIZENGHOU,FEIJIANZHIHEXUEGUANZHOUWEIXIBAOWAIJIZHIZENGDUO,CHENGXIANWEIXIBAOZENGDUO,BINGYOUDALIANGYANZHENGXIBAOJINRUN,

5. TANGNIAOBINGXIAOSHUSHENZANGZUZHIXINGTAIXUEDEBIANHUA

网络棋牌HERANSEXIANSHI,DMZUXIAOSHUSHENXIAOQIUFEIDAZENGSHENG,SHENXIAOQIUXIMOJIMOZENGHOU,SHENXIAOGUANKONGPAOBIANXING,BAOZHIKONGLIANG。

网络棋牌6. TANGNIAOBINGXIAOSHUGANZANGZUZHIXINGTAIXUEDEBIANHUA

网络棋牌JINGYOUHONGORANSEKEJIANTANGNIAOBINGXIAOSHUGANNEIZHIDIJIAODA,HONGSEZHIDIMINGXIAN,GANZANGKEJIANXIANZHUDEZHIZHICHENJI。

2020年2月26日 14:08
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